Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins.
نویسندگان
چکیده
A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.
منابع مشابه
Identification and characterization of a phase-specific, nuclear DNA binding protein from the dimorphic pathogenic fungus Histoplasma capsulatum.
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ورودعنوان ژورنال:
- Infection and immunity
دوره 70 4 شماره
صفحات -
تاریخ انتشار 2002